Question 1
Formation of duplex RNAs can regulate gene expression by:
◦ forming ribozymes that self-regulate.
◦ prevention of protein binding.
◦ triggering DNA methylation.
◦ recruiting the small ribosomal subunit.
◦ facilitating RNA hairpin formation.
Question 2
In RNAi, the miRNAs provided by RISC usually bind to:
◦ the 3′ UTR of a target mRNA.
◦ the promoter of a target gene.
◦ the 5′ UTR of a target mRNA.
◦ ribosomal RNAs, to inhibit translation.
◦ the coding region of a target mRNA.