Viral Titering-Plaque Assay

Author Question: Viral Titering-Plaque Assay (Read 539 times)

Creative Biogene

Newbie
Posts: 3

on: Aug 28, 2023

After the initial infection and application of the immobilizing overlay, individual plaques, or zones of cell death, will start to develop as viral infection and replication are constrained to the surrounding monolayer. Infected cells will continue the replication-lysis-infection cycle, further propagating the infection, leading to increasingly distinct and discrete plaques. According to the viral growth kinetics and host cell used, a visible plaque will ordinarily form within 2-14 days. After fixing and staining the infected cellular monolayer, plaques are counted so that titer viral stock samples in terms of plaque forming units (pfu) per milliliter.

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