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| Description: Genomic DNA Fragmentation into appropriate size by partial digestion or shearing — 1—20 kb 100—200 kb Ligation Ligation to vector to vector BAC Selectable marker Plasmid Bacterial origin (ori) \ 1 Transformation into E. coli 1 Each colony is an independent clone. All sequences originally present in the genomic DNA should be represented equally in the library.
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