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Description: The cloned gene encoding the F1b subunit was modified by adding a sequence that encodes an oligohistidine sequence that binds to a nickel-coated bead (Ni-NTA coated bead) in the conformation shown. Thus, the complex was immobilized on the bead and attached to a glass coverslip. Streptavidin is a protein used to couple fluorescent-tagged actin to the subunit. Picture Stats: Views: 302 Filesize: 100.88kB Height: 428 Width: 422 Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=34587 |