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The experimental system that permits observation of rotation in the F1 component of F1F0 ATP synthas

The experimental system that permits observation of rotation in the F1 component of F1F0 ATP synthas
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Description: The cloned gene encoding the F1b subunit was modified by adding a sequence that encodes an oligohistidine sequence that binds to a nickel-coated bead (Ni-NTA coated bead) in the conformation shown.

Thus, the complex was immobilized on the bead and attached to a glass coverslip.

Streptavidin is a protein used to couple fluorescent-tagged actin to the subunit.

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