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| Description: In blue-white selection procedures, DNA inserted into multiple cloning site of a plasmid disrupts the lacZ gene so that bacteria containing recombinant DNA are unable to metabolize X-gal, resulting in white colonies that allow direct identification of bacterial colonies carrying cloned DNA inserts. IacZ . / gene Multiple _ . ' cloning site Ampuclllm realslance (amp“) 1. Multiple cloning site of plasmid * is out with restriction enzyme. 3' 5, IacZ gene 5, / IacZ gene 2. DNA to be cloned cut with same restriction enzyme. 3.: 5' ‘ 3. DNA ligose joins together plasmid DNA and DNA to be cloned to create recombinant plasmid. IacZ gene DNA insertion / disrupts IacZ gene / IacZ gene 4. Transform bacteria with plasmids Grow cells on media t with amplclllin and X-gal. Bacteria with nonrecombinanl plasmids are blue Bacteria with recombinant plasmids are white Picture Stats: Views: 195 Filesize: 122.76kB Height: 1056 Width: 618 Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=44420 |