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| Description: Two-dimensional gel electrophoresis (2DGE) is a useful method for separating proteins in a protein extract from cells or tissues that contains a complex mixture of proteins with different biochemical properties. 1m Dimension: Load protein sample omo an isoelectric focusing tube gel. Electrophoresis separates proteins according to their isoelectril: point. where their net charge is zero compared to the pH of the get pH 4.0 pH 4.0 Proteins pH 10.0 pH 10.0 2"" Dimension: Rotate lube gel 9n' and place onto an SDS-paiyacrylamide gel (SUSPAG E). Electrophoresis separates proteins according to mass Stained gel shows proteins as a series (molecular weight in of spots separated by isoelociric point kliodaltnns, kDa) and molecular mass wn ; a. 2» ' “-"' ”’m SOS-PAGE ‘ .m - _ firm" . :4 L m . .. 4 \ mas/g... um”. “I 4.: sn 5: m u mi 1! m w Picture Stats: Views: 192 Filesize: 192.01kB Height: 1056 Width: 788 Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=45744 |