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DNA Cloning

DNA Cloning
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Description: An example of cloning. Here, a fragment of chromosomal DNA is inserted into a plasmid.

A A restriction enzyme cuts
a specific base sequence in chromosomal DNA and in
a plasmid cloning vector.

B A fragment of chromosomal DNA and the plasmid base-pair at their sticky ends. DNA ligase joins the two pieces of DNA.

C The recombinant plasmid is inserted into a host cell. When the cell multiplies, it makes multiple copies of the plasmids.

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Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=48607
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