Question 1
In the case of Jesse Gelsinger, a young man who died during gene therapy, the therapy involved delivery via a viral vector. Was this germ-line or somatic therapy?
◦ somatic
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Question 2
In the pUC plasmids, a polylinker region that includes multiple restriction sites is embedded in the
lacZ component. If a sequence of interest is inserted into this region, what is the effect on
lacZ?
◦ inactivation
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