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Author Question: Suppose a researcher is interested in using the CRISPR-Cas9 system to knock out the ... (Read 400 times)

codyclark

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on: Jul 15, 2019
Suppose a researcher is interested in using the CRISPR-Cas9 system to knock out the phosphofructokinase gene of a rat skin cell that codes for an enzyme that is critical for glycolysis to occur. The researcher added the Cas9 enzyme to the cell but forgot to add the guide RNA. What result do you think the researcher will observe?
◦ Glycolysis will proceed normally in the cell.
◦ Cas9 will specifically cut the phosphofructokinase gene.
◦ The phosphofructokinase enzyme will be expressed but will have lower activity.
◦ There will be mutations in the phosphofructokinase gene.
Campbell Biology: Concepts & Connections ¦ Taylor, Simon, Dickey, Hogan, Reece ¦ 9th Edition
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Marked as best answer by codyclark on Jul 15, 2019

LVPMS

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Reply #1 on: Jul 15, 2019
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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codyclark

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Reply #2 on: Jul 15, 2019
Thank you!


LVPMS

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Reply #3 on: Jul 15, 2019
Always glad to help...


Alexandria Scalzo

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Reply #4 on: May 4, 2020
thank you
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