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Purification of the desired protein by chromatography

Purification of the desired protein by chromatography
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Description: Purification of the desired protein by chromatography is the result of interactions between the proteins in the cell lysate and the matrix within the column.

The more strongly a protein interacts with the matrix, the later it will elute from the column.

Proteins are generally detected by UV absorbance at 280 nm as they elute from the column.

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Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=33927
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