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| Description: Detection of one protein in a complex mixture is shown. Steps 1–5 show a close-up view of a single well. In step 5 color develops in the well due to the action of the enzyme linked to the 2° antibody. The enzyme causes a chromogenic substrate to change color. In step 6, the entire 96-well plate is analyzed (each circle represents one of the 96 wells). Those wells with color are presumed to contain the target antigen. Picture Stats: Views: 227 Filesize: 185.9kB Height: 875 Width: 325 Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=34146 |