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The indirect ELISA assay

The indirect ELISA assay
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Description: Detection of one protein in a complex mixture is shown.

Steps 1–5 show a close-up view of a single well.

In step 5 color develops in the well due to the action of the enzyme linked to the 2° antibody.

The enzyme causes a chromogenic substrate to change color.

In step 6, the entire 96-well plate is analyzed (each circle represents one of the 96 wells). Those wells with color are presumed to contain the target antigen.

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