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Fragmentation of l bacteriophage DNA with restriction endonucleases EcoRI or BamHI

Fragmentation of l bacteriophage DNA with restriction endonucleases EcoRI or BamHI
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Description: a. Experimental determination of fragmentation patterns, resulting from enzymatic digestion of the 48.5 kb linear DNA molecule from phage l.

Restriction digests are subjected to agarose gel electrophoresis, and the fragments are visualized under ultraviolet light after staining the gel with ethidium bromide. Note that fragments with very similar sizes form but one band on a gel.

b. Maps of cleavage sites for each enzyme on the DNA molecule.

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