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The Klenow fragment of E. coli DNA polymerase I

The Klenow fragment of E. coli DNA polymerase I
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Description: a-Carbon backbone representation of the Klenow fragment complexed with DNA, in an editing configuration.

That is, the 3’ end of the growing strand (light blue) is bound at the 3’ exonuclease active site of the enzyme (yellow).

The locations of the polymerase and 3’ exonuclease active sites have been identified by site-directed mutagenesis.

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Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=34937
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