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Action of type I and type II topoisomerases, as shown by gel electrophoresis

Action of type I and type II topoisomerases, as shown by gel electrophoresis
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Description: Lane 1 shows a relaxed circular DNA.

Lane 2 shows the pattern from treatment of supercoiled DNA with type I topoisomerase.

Lanes 3–5 show relaxed circles treated with DNA gyrase, a type II topoisomerase, for different lengths of time.

Note that more different topoisomers can be seen in topoisomerase I reaction mixtures, as expected if changes in the linking number (L) occur in units of 1, whereas gyrase changes L in units of 2.

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