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Preparation of a 5S rRNA gene for transcription

Preparation of a 5S rRNA gene for transcription
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Description:
At least the three protein factors shown, plus RNA polymerase III, must assemble on the gene before transcription can occur.

TFIIIA must bind to the gene before factors TFIIIC and TFIIIB can bind.

Once the stable complex II has been formed, it will recycle with pol III to produce many RNA copies.

An excess of 5S rRNA will form a complex with TFIIIA, inhibiting further transcription.

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