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| Description: Cleavage with EcoRI S’mc i 3'J-I-Lc.T.T_A-A | Fragments with complementary sticky ends Cleavage with EcoRI A—A-T-T-Emy ¢J_|.J.5r Annealing allows recombinant DNA molecules to form by complementary base pairing. The two strands are not covalently bonded as indicated by the nicks in the DNA backbone. DNA Iigase seals the nicks in the DNA backbone, covalently bonding the two strands.
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