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Cloning with a plasmid vector

Cloning with a plasmid vector
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Description: Cloning with a plasmid vector involves cutting both plasmid and the DNA to be cloned with the same restriction enzyme

   Plasmid vector is removed from bacterial cell and out with a restriction enzyme    u Host cell ‘ chromosome The two DNA: are ligated to form a recombinant molecule troduction into bacterial ‘— 35'     ¢ DNA to be cloned is cut with the same restriction enzyme       ost cells by transformation  
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Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=44419
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