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| Description: . Colonies of the library are overlaid with a DNA-binding membrane such as nylon i Colonies transferred . Colonies are transferred to 75/ ‘0 membrane membrane, then lysed, and DNA is denatured . Membrane is placed in a heat-sealed bag with a solution containing the labeled probe; the probe hybridizes with denatured DNA from colonies . Membrane is rinsed to remove excess probe, then dried; X-ray film is placed over the filter for autoradiography or Film — —’ chemiluminescence signal from the probe recorded with a d'9"a' camera Hybridization of the . Using the original plate, cells are ‘ probe to one picked from the colony that colony from the hybridized to the probe , original plate is 1 indicated by a spot 3‘ on the X-ray film or i ‘ fluorescent spot on . Cells are transferred to a medium a digital image for growth and further analysis
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