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| Description: A 75. coli grown in 15N-labeled medium - E. coli DNA becomes uniformly labeled with 15N in nitrogenous bases Generation 0 Generation I Generation ll Generation Ill —> —> / —> —> 15N-labeled Cells Cells Cells E. coli added replicate replicate a replicate to ”N medium once in second time a third time _ 1"N in 14N in 1"N Gravitational force DNA extracted and centrifuged in gradient —» “3i WET; «Fl lung?‘ ‘5N/‘5N ‘5N/”N "NI”N ‘SN/"N “N/”N 15N/“‘N l
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