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| Description: In the Southern blotting technique, samples of the DNA to be probed are cut with restriction enzymes and the fragments are separated by gel electrophoresis. i 2 3 l. DNA samples cut with — — — restriction enzymes are loaded on ugerese gel lor electrophoresis Lane 1: DNA size markers Lane NA cut with restriction enzyme A Lane . NA cut with restriction enzyme 5 2. DNA is separaled by electrophoresis l 2 3 Weight _ Pa er towels I p __ DNA-binding liner .- - I DNA ls denatured _ —- Gel is placed "' one on ewlck _ I u P 9 Gel Wick (sponge) Butter a. DNA-binding liner, paper towels. and weight are placed on gel; buffer passes upward lhrough sponge by capillary action, lranslerring DNA lragrrrems to lilter Radioactive or nonradioacuvely labeled probe / 4. The liller is placed in heat-sealed bag or a tube with solulien containing labeled probe; probe hybr es with complementary sequences l _> _ — \\ _ _ 5. Bound probe All size markers appear because deleoleo by film or they are labeled; in lanes 2 PW” slallal captured and 3, only those bands that hybridize Wilh fl digi‘fl' came“ with probe are visible Picture Stats: Views: 206 Filesize: 189.8kB Height: 1056 Width: 883 Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=45665 |