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Mass spectrometry for identifying an unknown protein isolated from a 2D gel

Mass spectrometry for identifying an unknown protein isolated from a 2D gel
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Description: 1 2D gel it run a med inm small peptide hagmenls using n protease such I: Iryp: speclmmelry to produce mass-In-charge Subiect he e mgmems lo muss l (In/z) aplen- zoo Halalive auunaanu s k. 2m son 1000 Compare m/I spectra lor unknown pmlein to a nmwomlca ant-hm of M 5:12:11: var known peptides. A spectrum much would menury ma pepuae uquenu aim: unknown pmein. 200 E a = = a m .2 .5 a 1 VB 0 .LL ZED Sol] 1000
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