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| Description: In this example, a radioactive probe helps identify a colony of bacteria that host a targeted sequence of DNA. A Individual bacterial cells from a DNA library are spread over the surface of a solid growth medium. The cells divide repeatedly and form colonies—clusters of millions of genetically identical descendant cells. B A piece of special paper pressed onto the surface of the growth medium will bind some cells from each colony. C The paper is soaked in a solution that ruptures the cells and releases their DNA. The DNA clings to the paper in spots mirroring the distribution of colonies. D A probe is added to the liquid bathing the paper. The probe hybridizes (base-pairs) with the spots of DNA that contain complementary base sequences. E The bound probe makes a spot. Here, one radioactive spot darkens x-ray film. The position of the spot is compared to the positions of the original bacterial colonies. Cells from the colony that made the spot are cultured, and the DNA they contain is harvested. Picture Stats: Views: 115 Filesize: 207.43kB Height: 1171 Width: 500 Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=48609 |