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Genetic dissection of methionine biosynthesis pathway

Genetic dissection of methionine biosynthesis pathway
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Description: (a) Growth of a wild-type strain and four independent met- mutant strains on minimal medium and various supplemented minimal media. For each mutant, the compound that accumulates is the one that immediately precedes the point of blockage. (b) The order of intermediate compounds in the methionine biosynthesis pathway and the step blocked in each met- mutant strain(a) Growth of a wild-type strain and four independent met- mutant strains on minimal medium and various supplemented minimal media. For each mutant, the compound that accumulates is the one that immediately precedes the point of blockage. (b) The order of intermediate compounds in the methionine biosynthesis pathway and the step blocked in each met- mutant strain

(a) Experimental data Growth Medium Compound Mutant Minimal Minimal+ Minimal+ Minimal+ Minimal+ accumulating strain medium cysteine cystathionine homocysteine methionine in mutant Control prototroph + + + + + None Met 1 -- — -— + Homocysteine Met 2 — — — + + Cystathionine Met 3 -— — + + + Cysteine Met 4 — + + + + Homoserine (b) Order of intermediates in pathway Met 4 Met 3 Met 2 Met 1 l l l l Homoserine—> Cysteine—> Cystathionine—> Homocysteine—> Methionine
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