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| Description: a) Genetic complementation of two lysis-defective phage mutants occurs when the mutants carry mutations of different genes. Genetic complementation is revealed by the formation of many wild-type plaques on K12 () bacteria. (b) No complementation occurs in lysisdefective mutants that carry mutations of the same genea) Genetic complementation of two lysis-defective phage mutants occurs when the mutants carry mutations of different genes. Genetic complementation is revealed by the formation of many wild-type plaques on K12 () bacteria. (b) No complementation occurs in lysisdefective mutants that carry mutations of the same gene (a) Complementation of mutations in different genes Mutation Mutation rII locus_ x - l l l l Viral A B A B products: defective functional functional defective E. coli K12 (A) lawn I Wild-type T4 plaques During simultaneous infection, complementation occurs because functional forms of both A and 8 proteins are present. (b) No complementation of mutations in the same genes Mutation Mutation rnnocus— x — l i l l Viral A B A B products: defective functional defective functional E. coli K12 (A) lawn No plaques During simultaneous infection, no complementation occurs because no functional A proteins are present. Mutation Mutation rlllocus— x — l i l l Viral A B A B products: functional defective functional defective / E. coli K12 (A) lawn-L— During simultaneous infection, no complementation occurs because no functional B proteins are present. I} No plaques Picture Stats: Views: 90 Filesize: 752.78kB Height: 2921 Width: 1428 Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=49102 |