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| Description: (a) Replication of each fragment terminates with the addition of a ddNTP. Nucleotides of the newly synthesized ″sequenced strand″ are read off the gel. The 5-to-3 polarity of the sequenced strand corresponds to the smaller-to-larger fragment-length direction. The “inferred strand” is the complementary DNA strand, and it is antiparallel to the sequenced strand. (b) A photograph of a dideoxy sequencing gel with a segment of the sequence read
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