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| Description: a £1 5 Temperature-sensitive ‘ I cdc2 mutants of ‘ g I Schizosaccharomyces i g I pombe 3 \ o Transform with S. pombe cDNA expression library designed so that the cDNA sequences are transcribed and translated in the host \ ‘: CDC2- -containing plasmid M 3 @ Each 5. pombe cell "x: receives a different \} @ cDNA clone from the ’ library. l a Plate at 23°C. 1 9 Replica plate and grow at 36°C. Only colonies harboring a cDNA clone that can complement the ma mutant will grow at the restrictive temperature.
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