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| Description: Double—stranded RNA can be introduced by: Q Transfection or direct injection fd RNA % o s Q \firal—mediated I, introduction Vii % of dsRNA \, G Transgene with fix I inverted repeat Dicer /Transcription ~~~~~~~~~~~~~~~~~~ ‘ Transcript can dSRN x\§ \ d RNA form hairpin. 9 Cleavage of 5 dsRNA into 21- to 24base-Iong \\ S'RNA by DIcer \frgonaute e Cleavage of target mRNAs comple— mRNA mentary to siRNAs target by "slicer” activity Cleavage of Argonaute
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