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| Description: Strategy for generation of enhancer trap linesStrategy for generation of enhancer trap lines Endogenous regulatory element Enhancer trap DNA TATA box is used as a minimal promoter to recruit basal transcriptional machinery. EDI—Z Endogenous gene X 1 1 RNA — — «o W B-gal expression in pattern driven by endogenous regulatory element Reporter gene (B-gal) marker regulatory sequences. Selectable Randomly insert enhancer trap into genome via transposon or T-DNA vector. Endogenous gene X If enhancer trap disrupts coding region of gene, a loss-of-function allele is created. However, insertion of vector may occur 5’ or 3’ to a gene and still "trap" enhancers without causing a loss-of-function mutation. If enhancer trap DNA is inte- grated near endogenous regu- latory elements, the reporter gene will be expressed in a pattern driven by adjacent Picture Stats: Views: 27 Filesize: 317.25kB Height: 1320 Width: 2126 Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=49342 |