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| Description: Plasmid vector Insert DNA EcoRI Kpnl Kpnl _ Digest Digest with EcoRI. with Kpnl. / \ x/I \\\ ’ \ ’ \ ’ \ ‘ S’Eflh, h _5' 31‘ 3’CAr-g'fe-yfi-33' Fill in 5' 1 overhang i ' overhang i with DNA 1 g with exo- i polymerase. i ' nuclease. i f—3' 5'_‘ S'E-Ifi-Ejs' 4—9 3’” 3’gnflng5' Combine fragments. Recombinant vector I \
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