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| Description: .In the chemical structures, red type traces the fate of the two carbon atoms that enter the cycle via acetyl CoA (step 1), and blue type indicates the two carbons that exit the cycle as CO2 in steps 3 and 4. (The red labeling only goes through step 5, but you can continue to trace the fate of those carbons.) Notice that the carbon atoms that enter the cycle from acetyl CoA do not leave the cycle in the same turn. They remain in the cycle, occupying a different location in the molecules on their next turn after another acetyl group is added. As a consequence, the oxaloacetate that is regenerated at step 8 is composed of different carbon atoms each time around. All the citric acid cycle enzymes are located in the mitochondrial matrix except for the enzyme that catalyzes step 6, which resides in the inner mitochondrial membrane. Carboxylic acids are represented in their ionized forms, as OCOO?, because the ionized forms prevail at the pH within the mitochondrion. For example, citrate is the ionized form of citric acid.
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