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| Description: The restriction enzyme binds to a specific sequence in both the chromosomal and the vector DNA. It then cleaves the DNA backbones, producing DNA fragments. The complementary single-stranded ends of the DNA fragments hydrogen bond with each other. The enzyme DNA ligase then covalently links these fragments, producing a recombinant vector.
Picture Stats: Views: 1598 Filesize: 123.42kB Height: 889 Width: 850 Source: https://biology-forums.com/index.php?action=gallery;sa=view;id=586 Keywords: Step 2 of gene cloning: The actions of a restriction enzyme and DNA ligase to produce a recombinant |