Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to
A) insert the fragments cut with restriction enzyme X directly into the plasmid without cutting the plasmid.
B) cut the plasmid with restriction enzyme X and insert the fragments cut with restriction enzyme Y into the plasmid.
C) cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.
D) cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X.
E) cut the plasmid with restriction enzyme X and then insert the gene into the plasmid.
Overview of points of control for gene regulation in (a) prokaryotes and (b) eukaryotes
Potential applications of embryonic and tissue specific adult stem cell in cellular and gene theraph
One version of a bump cap is a molded plastic insert worn inside a regular cloth cap.
Mediator as a bridge between gene-specific regulatory factors and the general transcription
Gene products that are essential to mitochondrial function
Experimentally acquired evidence for gene annotation