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Author Question: What is the mechanism for the three-part differential found in older Coulter machines or those ... (Read 14 times)

pragya sharda

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on: Jun 25, 2018
What is the mechanism for the three-part differential found in older Coulter machines or those designed for smaller laboratories?
 
  a. Volume, conductivity, scatter
  b. Multiangle polarized scatter separation
  c. Peroxidase staining and light scatter
  d. Impedance after cytoplasm shrinkage

Question 2

How do CELL-DYN instruments differentiate red cells and platelets from one another?
 
  a. Differences in forward scatter using optical light scatter principle
  b. Use of a unique reagent that selectively lyses red cells while preserving platelets
  c. Differences in volume (fL) using electrical impedance principle
  d. Use of a unique reagent that selectively lyses platelets while preserving red cells


Zack0mack0101@yahoo.com

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Reply #1 on: Jun 25, 2018
Answer to Question 1

ANS: D
Older Coulter instruments use a special white blood cell (WBC) lysing agent that causes differential shrinkage of white cells, allowing the different white cells to be counted and sized based on their impedance.

Answer to Question 2

ANS: C
CELL-DYN instruments use electrical impedance for counting and discriminating red cells and platelets. Pulses between 1 and 35 fL are included in the initial platelet data, whereas pulses greater than 35 fL are counted as red cells.


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