Author Question: Which of the following, using Wright stain, is a feature of normal cells as they mature? a. ... (Read 18 times)

fagboi · **on:** Jun 25, 2018

Which of the following, using Wright stain, is a feature of normal cells as they mature?

a. Overall size increases
b. Nucleoli become more prominent
c. Nuclear chromatin becomes more condensed
d. Cytoplasmic basophilia increases

Question 2

Which of the following is a morphologically identifiable precursor cell using Wright stain?

a. Promyelocyte
b. Colony-forming uniterythrocyte (CFU-E)
c. Colony-stimulating factorgranulocyte-monocyte (CSF-GM)
d. HSC

reelove4eva · **Reply #1 on:** Jun 25, 2018

Answer to Question 1

ANS: C
Nuclear chromatin is initially diffuse or dispersed; as a cell matures, the chromatin undergoes a process of condensing and stains more intensely with Wright stain. The overall cell size decreases; nucleoli are no longer visible if even present, and cytoplasmic basophilia, present because of the high concentration of RNA in a young cell, decrease.

Answer to Question 2

ANS: A
Only the promyelocyte, which is an early granulocyte (neutrophil), can be identified using a Wright stain. None of the stem cells (CFU-E, CSF-GM, HSC) can be identified by morphology. The stem cells are functionally defined by their behavior in vitro using specific colony culture techniques. These require special growth media and specific cytokine (or cytokines). They can be identified using immunophenotypic techniques combined with flow cytometry.