Author Question: What can increase the resolution of an object under the microscope? a. Lowering the condenser ... (Read 64 times)

melly21297

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What can increase the resolution of an object under the microscope?
 
  a. Lowering the condenser
  b. Increasing the combined magnification provided by the ocular and objective lenses
  c. Enlarging the numeric aperture of the objective lens
  d. Adjusting the focus controls

Question 2

Where should the condenser be located when using the 100 oil objective?
 
  a. Lowest position
  b. Highest position
  c. Middle position
  d. Variable depending on amount of light desired



whitcassie

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Answer to Question 1

ANS: C
The numeric aperture determines resolution, which is the ability to distinguish two objects closely situated to one another as distinct entities. Increasing the numeric aperture increases resolution. Lowering the condenser changes the amount of light coming through the specimen. Magnification, whether from the ocular or objective lens, or the two combined, simply makes an image larger but cannot improve resolution. Adjusting the focus controls merely makes an image sharper, but this does not increase the resolution.

Answer to Question 2

ANS: B
The position of the condenser is set to maximize the amount of light coming through the object; its ideal position is set when adjusting for Koehler illumination, and its ideal position is in the highest position. It should not be changed to regulate light intensity.



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