Author Question: What are the advantages of tissue culture over other propagation techniques? What are its ... (Read 18 times)

Redwolflake15 · **on:** Jul 22, 2018

What are the advantages of tissue culture over other propagation techniques? What are its disadvantages?

What will be an ideal response?

Question 2

Outline the basic steps that would be followed in tissue culture.

What will be an ideal response?

rleezy04 · **Reply #1 on:** Jul 22, 2018

Answer to Question 1

Advantages: You can produce a plant that is free of viruses, and it enables mass production of a cultivar from a relatively small amount of parent material.
Disadvantages: The disadvantage is that it is technologically complex.

Answer to Question 2

a. Remove disinfected meristem from plant

b. Place it in a test tube of growing medium and close with sterile cotton

c. Wait for the explant to increase in size

d. Divide the explant into pieces and transfer them to new test tubes.

e. Wait for the new plants to grow and establish roots and shoots

f. Transfer the small plantlets to pots

g. Transfer the plants to outdoor growing conditions.