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Author Question: The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated ... (Read 69 times)

rachel9

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on: Mar 26, 2020
The PCR (polymerase chain reaction) protocol that is currently used in laboratories was facilitated by the discovery of a bacterium called Thermus aquaticus in a hot spring inside Yellowstone National Park, in Wyoming. This organism contains a heat-stable form of DNA polymerase known as Taq polymerase, which continues to function even after it has been heated to 95°C. Why would such a heat-stable polymerase be beneficial in PCR?
◦ Each cycle includes a "hot" denaturation phase (95°C), which serves to sterilize the culture.
◦ Each cycle includes a "hot" denaturation phase (95°C), which activates the Taq polymerase.
◦ Each cycle includes a "hot" saturation phase (95°C), which allows the primers to anneal to the target DNA.
◦ Each cycle includes a "hot" denaturation phase (95°C), which separates the hydrogen bonds that hold the strands of the template DNA together.
◦ More than one of the above are correct.
Essentials of Genetics ¦ Klug ¦ 9th Edition
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Marked as best answer by rachel9 on Mar 26, 2020

ttt030911

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Reply #1 on: Mar 26, 2020
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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rachel9

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Reply 2 on: Mar 26, 2020
:D TYSM

smrtceo

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Reply 3 on: Yesterday
Excellent

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