A competent bacterial strain that has the genotype a- b- c+ is transduced with a P1 phage carrying a fragment of donor chromosome with the a+ b+ c- alleles. The following number of transductants were found after screening for the selectable marker a+.
Transductant Genotype | Number |
a+ b- c+ | 160 |
a+ b+ c+ | 360 |
a+ b+ c- | 460 |
a+ b- c- | 20 |
Which of the following conclusions is false?
◦ The transduction frequency between
a and
b is 82%.
◦ The transduction frequency between
a and
c is 48%.
◦ Gene
a and gene
b are closer together than gene
a and gene
c.
◦ The gene order is
acb.
◦ The selected marker screen will inhibit growth of transductants with the genotype
a- b+ c-.