What defines the end of a eukaryotic gene?
◦ A 3' UTR of at least 25 nucleotides recruits an RNase that cleaves the pre-mRNA.
◦ There is no clearly defined end to eukaryotic genes, unlike for bacterial genes.
◦ A stem-loop structure in the transcriptional terminator region stalls the RNA polymerase.
◦ Presence of a stop codon leads to RNA polymerase stalling and ceasing transcription.
◦ Presence of a polyadenylation signal sequence leads to cleavage of the pre-mRNA.