Which of the following statements is true regarding enzyme multiplied immunoassay technique (EMIT)?
A. If analyte is present the activity of the enzyme, glucose-6-phosphate dehydrogenase is inhibited by the binding of antibody to the antigen conjugated with enzyme.
B. If no analyte is present the activity of the enzyme, glucose-6-phosphate dehydrogenase is inhibited by the binding of antibody to an antigen conjugated with enzyme.
C. If no analyte is present the activity of the enzyme, fructose-6-phosphate dehydrogenase is inhibited by the binding of antibody to an antigen conjugated with enzyme.
D. If no analyte is present the activity of the enzyme, alkaline phosphatese glucose-6-phosphatase dehydrogenase is enhanced by the binding of antibody to the antigen conjugated with enzyme.
Question 2
Affinity is described as which of the following?
A. The overall strength of binding of an antibody to an antigen and includes the sum of the binding affinities of all the individual binding sites on the antibody
B. Another substance that is capable of producing antibodies
C. The thermodynamic quantity defining the energy of interaction of a single antibody-binding site and its corresponding epitope on the antigen
D. A chemical substance capable of inducing an immune response
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Stereospecificity conferred by an enzyme