Tartrate-resistant acid phosphatase is strongly positive in which ...

[Pea0909berry]

Tartrate-resistant acid phosphatase is strongly positive in which of the following cells?
 
  a. Myeloblasts
  b. Hairy cells
  c. Granulocytes
  d. Lymphocytes

Question 2

The bone marrow of a patient with acute leukemia is stained with the periodic acidSchiff (PAS) stain. When this stain is evaluated, segmented neutrophils show diffuse pink staining. Select the correct interpretation.
 
  a. The patient has acute lymphoblastic leukemia.
  b. The patient has acute myeloid leukemia.
  c. The stain did not work.
  d. No conclusion can be reached; blast cells must be examined.

[Chocorrol77]

Answer to Question 1

ANS: B
Hairy cells are positive for acid phosphatase when tartrate is added to the incubation mixture. They uniquely contain an abundance of acid phosphatase isoenzyme 5; all other isoenzymes of acid phosphatase, found in almost all other blood cells, are inhibited in the presence of tartrate. At least two or more cells should contain >40 red granules to call the stain positive.

Answer to Question 2

ANS: D
The fact that mature neutrophils stain positive has no diagnostic significance in a patient with acute leukemia. Only examination of the blast cells is useful.

[Pea0909berry]

TYSM

[momolu]

Thanks for the timely response, appreciate it