A Hodgkin lymphoma patient is admitted for treatment. What would ...

[student77]

A Hodgkin lymphoma patient is admitted for treatment. What would be the benefit in performing an EBV-DNA test on him?
 
  a. It would identify the cause of the Hodgkin lymphoma.
  b. Because the patient is immunocompromised, it would identify an opportunistic pathogen.
  c. It would help monitor the efficacy of his treatment.
  d. It would help rule out other types of lymphomas.

Question 2

Name and compare two molecular methods used to detect mutations associated with sickle cell disease.
 
  What will be an ideal response?

[Edwyer]

Answer to Question 1

Correct Answer: C

Answer to Question 2

Answer: Two molecular methods that detect the mutation associated with sickle cell disease are Southern blot analysis and PCR. Southern blot: Restriction endonucleases cut the chromosomal DNA of the suspected sickle patient at a specific nucleotide base to produce smaller fragments of DNA. A probe specific for the gene responsible for SCD (HBB gene) is applied and targeted to the specific sequence. If the aberrant gene is present, it will show up as a band on 2D-gel. (Qualitative: present or absent.)
PCR: Similar procedure to Southern blot analysis, but at the end, the targeted DNA sequence is amplified a billionfold.