Explain how bone marrow analysis helps establish an AML ...

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Explain how bone marrow analysis helps establish an AML diagnosis.
 
  What will be an ideal response?

Question 2

List the common cytochemical stains used in the detection of AML, and predict their results in AML.
 
  What will be an ideal response?

[cat123]

Answer to Question 1

Answer: The first step in bone marrow analysis is to establish the overall cellularity of the bone marrow. Next, the number of erythroblasts are counted and expressed as a percentage of the total number of nucleated cells in the marrow. A cutoff of 50 is considered standard. If erythroblasts comprise <50 of all nucleated cells, the nonerythroid precursors (i.e., myeloblasts and monoblasts) are then counted. If erythroblasts comprise >50 of all nucleated cells, a diagnosis of acute erythroleukemia (AEL) is suspected. If myeloblasts and/or monoblasts are >20 of the total count, AML is strongly suspected.

Answer to Question 2

Answer:
Myeloperoxidase (MPO): black color; positive
Sudan Black B (SBB): black color; positive
Nonspecific esterase (NSE): blue color; negative in myeloid AML; positive in myelomonocytic or monocytic AML
Specific esterase (SE): orange color; positive
Periodic Acid Schiff (PAS): coarse granular pattern; erythroblasts (AML-AEL).

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TYSM

[frankwu0507]

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