After reading the paragraphs below, answer the questions that follow.
You recently sprayed your apartment with insecticide to remove an infestation of cockroaches. In your kitchen, you noticed some roaches lying on their backs twitching after being exposed to the insecticide. This aroused your curiosity, so you decided to investigate exactly how the insecticide works on the nervous system.
In your research, you discover that the insecticide you used contains a permanent acetylcholinesterase inhibitor. Acetylcholine is a neurotransmitter that stimulates skeletal muscle to contract (including insect muscles). Acetylcholinesterase removes acetylcholine from the synapse after the signal is received. Exposure to high pesticide concentrations has a similar effect on humans, which can also be caused by exposure to the nerve gas Sarin and other chemical agents.
Why did the insecticide cause uncontrollable twitching in the roaches?
◦ Acetylcholine was released, but the insecticide prevented it from diffusing across the synapse.
◦ Acetylcholine was released, but the insecticide prevented it from binding to the receptor sites of the postsynaptic neurons.
◦ The insecticide prevented acetylcholinesterase from being removed from the synapse.
◦ The insecticide caused continuous stimulation of the muscles.
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