What is one major limitation of two-dimensional gel electrophoresis (2DGE)?
◦ It is extremely costly to execute in a typical molecular biology laboratory.
◦ 2DGE can be run only on nucleic acids.
◦ When products are separated, they tend to leach out of the gel matrix.
◦ Only the most abundant products are detected.
◦ 2DGE is useful only in separating eukaryotic gene products.