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Author Question: In TILLING, a population of inbred organisms is mutagenized to saturation. DNA from mutagenized ... (Read 98 times)

lidoalex

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on: Jan 12, 2022

Question 1

Design an experiment that uses enhancer trapping to drive the expression of a gene of interest.

Question 2

In TILLING, a population of inbred organisms is mutagenized to saturation. DNA from mutagenized lines is isolated and amplified by PCR. PCR products are denatured and allowed to reanneal and then treated with an endonuclease. Why is the denaturation/re-annealing step important for the detection of mutant alleles?
Genetic Analysis: An Integrated Approach ¦ Sanders, Bowman ¦ 3rd Edition
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Marked as best answer by lidoalex on Jan 12, 2022

Carissamariew

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Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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lidoalex

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Reply 2 on: Jan 12, 2022
Thanks for the timely response, appreciate it

tanna.moeller

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Reply 3 on: Yesterday
Great answer, keep it coming :)

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