The final step in translation initiation in bacteria is:
◦ association of initiator tRNA with the 30S ribosomal subunit.
◦ association of mRNA with the 30S ribosomal subunit.
◦ 50S subunit binding to the 30S ribosomal subunit complex.
◦ stabilize free 50S ribosomal subunits to facilitate assembly into mature ribosome.
◦ disassociation of IF-3 from the 30S ribosomal subunit.