Question 1
The yeast two-hybrid assay is used to identify:
◦ protein-DNA interactions.
◦ protein-protein interactions.
◦ protein-RNA interactions.
◦ DNA-RNA interactions.
◦ DNA-histone interactions.
Question 2
Which of the following is thought to be the mechanism by which DNA-histone octamer contacts are broken during chromatin remodeling to allow the octamer to be repositioned?
◦ A recombination step driven by single-stranded DNA generated in a local region
◦ Helicase-catalyzed unwinding of the DNA
◦ Local ionic changes that disrupt the histone-DNA interactions
◦ ATP-driven twisting of the DNA on the surface of the nucleosome
◦ Enzymatic digestion and resynthesis of the DNA strand