This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Consider DNA sequencing experiments using 2',3'-dideoxyribonucleoside triphosphates. Which of the ... (Read 34 times)

lbcchick

  • Hero Member
  • *****
  • Posts: 550
on: Aug 23, 2018
Consider DNA sequencing experiments using 2',3'-dideoxyribonucleoside triphosphates. Which of the following is not correct?
 a. 2',3'-dideoxyribonucleoside triphosphates are added to the replicating strand by DNA polymerase.
  b. There are four types of 2',3'-dideoxyribonucleoside triphosphates used.
  c. The sequence of 2',3'-dideoxyribonucleoside triphosphates in the replicated DNA yields the DNA structure.
  d. DNA replication ceases when a 2',3'-dideoxyribonucleoside triphosphates is added to the replicating strand.

Question 2

Describe the difference between a 2',3'-dideoxyribonucleoside triphosphate and 2'-deoxyribonucleoside triphosphate.


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

leannegxo

  • Sr. Member
  • ****
  • Posts: 374
Reply #1 on: Aug 23, 2018
Answer to Question 1

c

Answer to Question 2

A 2'-deoxyribonucleoside triphosphate is one in which the 2' OH group is missing from ribose. A 2',3'-dideoxyribonucleoside triphosphate is one in which both 2' and 3' OH groups are missing from ribose. In DNA sequencing the significance of this removal is that DNA replication stops at this nucleotide since the 3' OH needed for the continued addition of nucleotides.


lbcchick

  • Member
  • Posts: 550
Reply 2 on: Aug 23, 2018
Wow, this really help

  • Member
  • Posts:
Reply 3 on: Yesterday
Gracias!

« previous next »
 

Did you know?

The Centers for Disease Control and Prevention has released reports detailing the deaths of infants (younger than 1 year of age) who died after being given cold and cough medications. This underscores the importance of educating parents that children younger than 2 years of age should never be given over-the-counter cold and cough medications without consulting their physicians.

Did you know?

Egg cells are about the size of a grain of sand. They are formed inside of a female's ovaries before she is even born.

Did you know?

Anesthesia awareness is a potentially disturbing adverse effect wherein patients who have been paralyzed with muscle relaxants may awaken. They may be aware of their surroundings but unable to communicate or move. Neurologic monitoring equipment that helps to more closely check the patient's anesthesia stages is now available to avoid the occurrence of anesthesia awareness.

Did you know?

Although puberty usually occurs in the early teenage years, the world's youngest parents were two Chinese children who had their first baby when they were 8 and 9 years of age.

Did you know?

Adolescents often feel clumsy during puberty because during this time of development, their hands and feet grow faster than their arms and legs do. The body is therefore out of proportion. One out of five adolescents actually experiences growing pains during this period.

The traits Mendel used in his experiments, and the results of the F1 and F2 generation crosses. The traits Mendel used in his experiments, and the results of the F1 and F2 generation crosses.
Experiments that showed DNA to be the genetic substance Experiments that showed DNA to be the genetic substance
Sanger Sequencing Sanger Sequencing
Nucleotides used in DNA sequencing reactions. Nucleotides used in DNA sequencing reactions.
Reading a dideoxy DNA sequencing gel Reading a dideoxy DNA sequencing gel
Paired-end shotgun sequencing strategy Paired-end shotgun sequencing strategy
Video: Neural regulation of blood pressure - baroreceptor and chemoreceptor reflexes

For a complete list of videos, visit our video library