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Author Question: Consider DNA sequencing experiments using 2',3'-dideoxyribonucleoside triphosphates. Which of the ... (Read 46 times)

lbcchick

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on: Aug 23, 2018
Consider DNA sequencing experiments using 2',3'-dideoxyribonucleoside triphosphates. Which of the following is not correct?
 a. 2',3'-dideoxyribonucleoside triphosphates are added to the replicating strand by DNA polymerase.
  b. There are four types of 2',3'-dideoxyribonucleoside triphosphates used.
  c. The sequence of 2',3'-dideoxyribonucleoside triphosphates in the replicated DNA yields the DNA structure.
  d. DNA replication ceases when a 2',3'-dideoxyribonucleoside triphosphates is added to the replicating strand.

Question 2

Describe the difference between a 2',3'-dideoxyribonucleoside triphosphate and 2'-deoxyribonucleoside triphosphate.


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leannegxo

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Reply #1 on: Aug 23, 2018
Answer to Question 1

c

Answer to Question 2

A 2'-deoxyribonucleoside triphosphate is one in which the 2' OH group is missing from ribose. A 2',3'-dideoxyribonucleoside triphosphate is one in which both 2' and 3' OH groups are missing from ribose. In DNA sequencing the significance of this removal is that DNA replication stops at this nucleotide since the 3' OH needed for the continued addition of nucleotides.


lbcchick

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Reply 2 on: Aug 23, 2018
Thanks for the timely response, appreciate it

amit

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Reply 3 on: Yesterday
Wow, this really help

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